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Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells
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Biochemical analysis of a caveolae-enriched plasma membrane fraction from rat liver

M Calvo1, C Enrich

  • 1Departament de Biologia Cel.lular, IDIBAPS, Facultat de Medicina, Universitat de Barcelona, Spain.

Electrophoresis
|November 18, 2000
PubMed

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The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
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The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the anterior...