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Kinetics of continuous starch hydrolysis in a membrane reactor
Paolucci-Jeanjean1, Belleville, Rios
1Laboratoire des Matériaux et des Procédés Membranaires (LMPM UMR 5635), ENSCM, 276 rue de La Galéra, 34097 cedex, Montpellier, France
Biochemical Engineering Journal
|November 18, 2000
Summary
This study investigates enzymatic starch hydrolysis kinetics in a continuous recycled membrane reactor (CRMR). A new equation models oligosaccharide production, accounting for enzyme activity decay over time in the CRMR system.
Area of Science:
- Biochemical Engineering
- Enzyme Kinetics
- Bioprocess Technology
Background:
- Previous research established batch reactor kinetics for Termamyl 120l enzymatic starch hydrolysis.
- Continuous recycled membrane reactors (CRMR) offer potential advantages for enzymatic processes.
Purpose of the Study:
- To investigate and model the kinetics of enzymatic starch hydrolysis in a CRMR.
- To develop a predictive equation for oligosaccharide production rates within the CRMR.
Main Methods:
- Utilized a continuous recycled membrane reactor (CRMR) setup.
- Collected kinetic data under various operating conditions.
- Proposed a mathematical model for oligosaccharide production rates.
Main Results:
- Developed an equation relating small oligosaccharide production rates (DP 1-5) to higher oligosaccharide concentrations.
- Identified a significant variation in enzyme activity over time within the CRMR.
- Incorporated an exponential decay function to accurately represent enzyme activity changes.
Conclusions:
- The proposed kinetic model effectively describes starch hydrolysis in a CRMR.
- Enzyme activity decay is a critical factor influencing hydrolysis kinetics in CRMRs.
- The findings provide a basis for optimizing CRMR design and operation for enzymatic starch hydrolysis.