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fbpABC gene cluster in Neisseria meningitidis is transcribed as an operon
1Department of Microbiology and Infectious Disease, University of Calgary, Calgary, Alberta, Canada T2N 4N1.
Infection and Immunity
|November 18, 2000
Abstract:
The neisserial fbpABC locus has been proposed to constitute a single transcriptional unit. To confirm this operonic arrangement, transcription assays using reverse transcriptase PCR amplification were conducted with Neisseria meningitidis. The presence of fbpAB and fbpBC transcripts obtained by priming cDNA synthesis with an fbpC-sequence-specific oligonucleotide indicates that fbpABC is organized as a single expression unit. The ratio of fbpA to fbpABC mRNA was approximately between 10- to 20-fold, as determined by real-time quantitative PCR.