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Updated: Aug 8, 2026
In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
Published on: May 16, 2011
In vitro adhesion of Staphylococcus epidermidis to intraocular lenses
M C García-Sáenz1, A Arias-Puente, M J Fresnadillo-Martinez
1Fundación Hospital de Alcorcón, Madrid, Spain. mcgarcias@fhalcorcon.es
Purpose:
To compare the in vitro adherence of slime-producing and non-slime-producing Staphylococcus epidermidis to different intraocular lenses (IOLs) to study the organism's contribution to postoperative endophthalmitis.
Methods:
Strains of slime-positive (ATCC 35984) and slime-negative (ATCC 12228) S epidermidis were used. The IOLs were made of poly(methyl methacrylate) (PMMA), PMMA with polypropylene haptics, silicone, hydrogel, acrylic, heparin-surface-modified (HSM) PMMA, and fluorine-surface-modified PMMA. The lenses were incubated overnight with bacteria, then sonicated and vortexed to separate the adhered bacteria. Quantitative cultures were performed and the results statistically analyzed.
Results:
Slime-negative strains of S epidermidis adhered to all IOLs but at a lower level than slime-positive strains. The most adherent lenses were acrylic with the positive strain and PMMA with the negative strain. The least adherent IOLs were PMMA with the positive strain and hydrogel with the negative strain. There were no significant differences between rigid and foldable lenses. Polypropylene was significantly more adherent than PMMA to the slime-positive strain. The acrylic and the HSM PMMA IOLs were significantly more adherent to the positive strain.
Conclusions:
In vitro, there were significant differences in bacterial adhesion among IOL materials. Slime-positive strains of S epidermidis were more adherent than slime-negative ones.
