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Related Experiment Videos

Streptokinase does not activate the complement system.

J Skopál1, M Vastag, L Varga

  • 1National Stroke Centre, Budapest, Hungary. skopal@opni.hu

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
|November 21, 2000
PubMed
Summary
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Different streptokinase preparations can cause hypotension due to complement activation. However, this study found that product contamination, not streptokinase itself, is the likely cause of complement system activation.

Area of Science:

  • Immunology
  • Pharmacology
  • Biochemistry

Background:

  • Streptokinase is a widely used thrombolytic medication.
  • Hypotension during streptokinase therapy is often linked to complement cascade activation.
  • Variability in patient response suggests differences in streptokinase product quality.

Purpose of the Study:

  • To investigate the cause of complement activation by different streptokinase preparations.
  • To determine if streptokinase itself or contaminants trigger the complement cascade.
  • To identify specific streptokinase products that do not activate the complement system.

Main Methods:

  • Measurement of soluble terminal complement complex (SC5b-9) levels in patients and normal subjects.
  • In vitro testing of four different streptokinase preparations (Streptase, Kabikinase, Calbiochem, Sigma) for complement activation.

Related Experiment Videos

  • Analysis of streptokinase purity using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE).

    • Main Results:

    • Four ischemic stroke patients treated with Streptase showed increased SC5b-9 levels.
    • Streptase, Kabikinase, and Calbiochem streptokinases significantly increased SC5b-9 in normal subjects.
    • Sigma streptokinase did not activate the complement system and showed a homogeneous band on SDS-PAGE.
    • Streptase, Kabikinase, and Calbiochem preparations were contaminated with albumin and other proteins.

    Conclusions:

    • Complement activation by streptokinase preparations is primarily due to product contamination, not the streptokinase molecule itself.
    • Sigma streptokinase, being free of contaminants, did not activate the complement system.
    • This finding has implications for the selection of safer thrombolytic agents and quality control in pharmaceutical manufacturing.