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Pyelonephritic potential of Pseudomonas aeruginosa in ascending mouse model
Abstract:
The suitability of female BALB/c and Swiss Webster (LACA) strains of mice for an ascending model of pyelonephritis for human uroisolate of Pseudomonas aeruginosa, was assessed. A fresh isolate of Ps. aeruginosa elaborating virulence factors like elastase, protease, phospholipase C, pyochelin and haemolysin was selected and introduced transuretherally in mice without any manipulation of the urinary tract. This isolate was able to colonize and persist in the renal tissue till day 12 post infection. Histopathologically, severe renal lesions i.e., abscesses formation was observed. The LACA strain was found to be more susceptible to infection with the selected Ps. aeruginosa isolate.
Insights
This study assessed mouse models for pyelonephritis caused by Pseudomonas aeruginosa. The LACA mouse strain proved more susceptible to infection, developing renal abscesses.
Area of Science:
- Microbiology
- Infectious Diseases
- Animal Models
Background:
- Pyelonephritis is a serious kidney infection often caused by uropathogenic bacteria.
- Pseudomonas aeruginosa is a significant opportunistic pathogen.
- Developing reliable animal models is crucial for studying pyelonephritis pathogenesis and treatment.
Purpose of the Study:
- To evaluate the suitability of BALB/c and Swiss Webster (LACA) mice for an ascending pyelonephritis model.
- To characterize the virulence of a specific human uroisolate of Pseudomonas aeruginosa.
- To compare the susceptibility of two mouse strains to Pseudomonas aeruginosa-induced pyelonephritis.
Main Methods:
- A virulent clinical isolate of Pseudomonas aeruginosa was used.
- The bacteria were introduced transurethrally into female mice.
- Mice were monitored for colonization, persistence, and histopathological changes in renal tissue.
- BALB/c and LACA mouse strains were compared for susceptibility.
Main Results:
- The selected Pseudomonas aeruginosa isolate colonized and persisted in renal tissue for up to 12 days post-infection.
- Severe renal lesions, including abscess formation, were observed histopathologically.
- The LACA mouse strain demonstrated higher susceptibility to the Pseudomonas aeruginosa infection compared to the BALB/c strain.
Conclusions:
- The LACA mouse strain is a suitable model for studying ascending pyelonephritis caused by this Pseudomonas aeruginosa uroisolate.
- The study highlights the virulence potential of the selected bacterial isolate.
- This model can be utilized for further research into pyelonephritis mechanisms and therapeutic interventions.