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Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
Published on: July 7, 2020
The Red Menace: Emerging Issues in Antimicrobial Resistance in Gram-Negative Bacilli.
1Medical Service 111(W), Veterans Affairs Medical Center, 10701 East Boulevard, Cleveland, OH 44106, USA.
Current Infectious Disease Reports
|November 30, 2000
Summary
Antibiotic resistance in Gram-negative bacilli is increasing, driven by complex fluoroquinolone resistance mechanisms and beta-lactamase production. Understanding these genetic factors is crucial for controlling infections.
Area of Science:
- Microbiology
- Infectious Diseases
- Pharmacology
Background:
- Gram-negative bacilli are a major cause of hospital-acquired infections in the US.
- Antimicrobial resistance in these pathogens is a growing concern, particularly for fluoroquinolone and beta-lactam agents.
Purpose of the Study:
- To review the mechanisms of resistance to fluoroquinolone and beta-lactam antibiotics in Gram-negative bacilli.
- To highlight the importance of understanding the genetic and biochemical basis of resistance for effective control strategies.
Main Methods:
- Literature review of recent research on antibiotic resistance mechanisms.
- Analysis of genetic and biochemical pathways involved in resistance phenotypes.
Main Results:
- Fluoroquinolone resistance involves target mutations, reduced outer membrane permeability, and active efflux.
- Beta-lactam resistance is mainly due to the production of beta-lactamases.
- Pseudomonas aeruginosa exhibits specific challenges with outer membrane permeability.
Conclusions:
- Emergence of resistance to fluoroquinolones and beta-lactams in Gram-negative bacteria is a significant clinical challenge.
- Understanding the genetic and biochemical mechanisms of resistance is essential for developing strategies to combat their spread and impact.
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