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Related Experiment Videos

Quantification of Staphylococcus aureus cell surface adhesins using flow cytometry.

N Mohamed1, L Visai, P Speziale

  • 1Department of Chemical and Biochemical Engineering, University of Maryland, Baltimore County, Baltimore, MD 21250, USA.

Microbial Pathogenesis
|November 30, 2000
PubMed
Summary

Staphylococcus aureus uses surface adhesins to bind host tissues. This study quantifies collagen and fibronectin adhesin levels, revealing distinct temporal expression patterns crucial for understanding infectious disease initiation.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Biochemistry

Background:

  • Bacterial adhesion to host extracellular matrix (ECM) is critical for initiating infectious diseases.
  • Staphylococcus aureus exhibits specific binding to ECM proteins like collagen and fibronectin.

Purpose of the Study:

  • To quantify collagen and fibronectin adhesin densities on Staphylococcus aureus surfaces.
  • To investigate the temporal expression patterns of these adhesins.
  • To present a novel method for bacterial adhesin quantification.

Main Methods:

  • Flow cytometry was employed to quantify adhesin densities.
  • Analysis focused on collagen and fibronectin adhesins on the bacterial surface.

Main Results:

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  • Quantification of collagen and fibronectin adhesin densities was successfully performed.
  • Observed temporal expression patterns of the two adhesins differed among strains studied.
  • Results align with previous findings on the transcription of relevant genes.

Conclusions:

  • A convenient and effective technique for quantifying bacterial adhesins was demonstrated.
  • This method aids in characterizing bacterial adhesion to ECM components.
  • Understanding adhesin expression is vital for studying infection progression.