Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative PCR.

R Jung1, K Soondrum, M Neumaier

  • 1Department for Clinical Chemistry, Medical Clinic, University Hospital Hamburg-Eppendorf, Hamburg, Germany.

Clinical Chemistry and Laboratory Medicine
|November 30, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical benefit of additional whole-exome sequencing over panel sequencing in an all-comer real-world molecular tumor board.

ESMO open·2025
Same author

Four-dimensional (4D) virtual simulation for predicting soft tissue support and vertical dimension of occlusion in full-arch implant therapy - A proof of concept.

Journal of dentistry·2025
Same author

Substance-Gain Extrusion Technique (SGET) in Combination With BOPT-Technique Description.

Journal of esthetic and restorative dentistry : official publication of the American Academy of Esthetic Dentistry ... [et al.]·2025
Same author

Simultaneous modulation of pulse charge and burst period elicits two differentiable referred sensations.

Journal of neural engineering·2024
Same author

Post-coronavirus disease 2019 polyneuropathy with significant response to immunoglobulin therapy: a case report.

Journal of medical case reports·2021
Same author

What do we need to obtain high quality circulating tumor DNA (ctDNA) for routine diagnostic test in oncology? - Considerations on pre-analytical aspects by the IFCC workgroup cfDNA.

Clinica chimica acta; international journal of clinical chemistry·2021
Same journal

Ethical evaluation of reflex and reflective testing in conformity with ISO 15189:2022 at the value-based laboratory medicine era.

Clinical chemistry and laboratory medicine·2026
Same journal

Emerging blood-based diagnostic strategies for tuberculosis.

Clinical chemistry and laboratory medicine·2026
Same journal

Homocitrulline and 3-year mortality in older hospitalised adults: an exploratory study.

Clinical chemistry and laboratory medicine·2026
Same journal

Impact of age partitioning on classification discordance in pediatric ferritin reference intervals.

Clinical chemistry and laboratory medicine·2026
Same journal

National implementation of LOINC: translation methodology and experience from the Polish laboratory terminology standardization project.

Clinical chemistry and laboratory medicine·2026
Same journal

Harmonization of measurement units: mission impossible or ethical imperative?

Clinical chemistry and laboratory medicine·2026
See all related articles

Quantitative polymerase chain reaction (qPCR) enables precise measurement of nucleic acid targets, overcoming limitations of traditional methods. This technology is crucial for medical diagnostics and genetic research, offering detailed cellular analysis.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Traditional molecular biology techniques like Northern and Southern blots require substantial nucleic acids from potentially heterogeneous samples.
  • Polymerase chain reaction (PCR) amplifies specific nucleic acid sequences but traditional methods lack quantitative correlation to initial target copy numbers.

Purpose of the Study:

  • To address the need for accurate quantification of specific nucleic acid targets in medical and research applications.
  • To highlight the development and utility of quantitative PCR (qPCR) techniques.

Main Methods:

  • Utilizing PCR-based techniques for specific amplification of nucleic acid sequences.
  • Employing real-time detection methods to monitor PCR product increment per cycle.

Related Experiment Videos

  • Integrating advanced tissue dissection and fluid fractionation techniques.

    • Main Results:

    • Quantitative PCR techniques provide accurate measurement of target nucleic acid quantities, unlike conventional PCR.
    • Real-time detection allows for precise monitoring of amplification during the PCR process.
    • New technologies facilitate detailed genetic analysis at the single-cell level and in complex biological samples.

    Conclusions:

    • Quantitative PCR is essential for applications such as viral load determination (e.g., HIV, HCV), gene dosage analysis, and gene expression profiling.
    • Real-time qPCR, combined with advanced sample preparation, enables sophisticated analysis of cellular genetics and gene usage, including pharmacogenomics.