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Related Experiment Videos

Prostaglandins regulate acid-induced cell-mediated bone resorption.

N S Krieger1, W R Parker, K M Alexander

  • 1Department of Medicine, Nephrology Unit, University of Rochester School of Medicine, Rochester, New York 14642, USA. Nancy_Krieger@urmc.rochester.edu

American Journal of Physiology. Renal Physiology
|November 30, 2000
PubMed
Summary

Metabolic acidosis stimulates bone resorption through prostaglandins. Inhibiting prostaglandin synthesis with indomethacin blocked this effect, indicating prostaglandins mediate acid-induced bone calcium loss.

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Area of Science:

  • Biochemistry
  • Bone Physiology
  • Acid-Base Balance

Background:

  • Metabolic acidosis causes bone calcium loss through dissolution and cell-mediated processes.
  • Acidosis increases prostaglandin synthesis in the kidney; prostaglandins are known bone resorption mediators.

Purpose of the Study:

  • To investigate if prostaglandins mediate acid-induced bone resorption.

Main Methods:

  • Neonatal mouse calvariae were cultured in neutral or acidic medium.
  • Indomethacin was used to inhibit prostaglandin synthesis.
  • Net calcium efflux and prostaglandin E(2) (PGE(2)) levels were measured.

Main Results:

  • Acidic medium increased net calcium flux and PGE(2) levels.

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  • Indomethacin inhibited both acid-induced calcium flux and PGE(2) production.
  • Net calcium flux directly correlated with PGE(2) levels; exogenous PGE(2) induced calcium flux.

    • Conclusions:

    • Acid-induced bone resorption is mediated by endogenous prostaglandin synthesis, particularly from osteoblasts.