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ComboScreen facilitates the multiplex hybridization-based screening of high-density clone arrays
D C Jamison1, J W Thomas, E D Green
1Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA. cjamison@informaxinc.com
Bioinformatics (Oxford, England)
|December 1, 2000
Summary
ComboScreen software facilitates high-throughput screening of bacterial artificial chromosome (BAC) clone arrays using multiplex hybridization. This tool aids in constructing physical maps and identifying clones for sequencing, streamlining molecular genetics research.
Area of Science:
- Genomics
- Molecular Biology
- Bioinformatics
Background:
- Physical mapping using bacterial artificial chromosomes (BACs) is crucial for genomic analysis and clone selection for sequencing.
- High-density clone array screening commonly employs DNA probe hybridization.
- Multiplex hybridization strategies reduce experimental effort but generate complex data requiring efficient analysis.
Purpose of the Study:
- To develop a computational tool for managing and analyzing multiplex hybridization data from high-density clone arrays.
- To streamline the process of screening bacterial clone libraries for physical mapping applications.
Main Methods:
- Development of a software program named ComboScreen.
- Implementation of organizational frameworks and analytical tools for high-throughput screening.
- Application of the software to construct mouse sequence-ready BAC contig maps.
Main Results:
- ComboScreen provides essential tools for analyzing multiplex hybridization screening data.
- The software facilitates efficient management and disambiguation of large datasets.
- Successful application in constructing mouse BAC contig maps demonstrates its utility.
Conclusions:
- ComboScreen is an effective tool for high-throughput screening of clone arrays via multiplex hybridization.
- The software simplifies the analysis of complex hybridization data, aiding physical map construction.
- ComboScreen supports molecular genetics applications, including sequencing-ready map development.