Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Patterns within protein/polyphosphoinositide interactions provide specific targets for therapeutic intervention.

C P Berrie1, M Falasca

  • 1Department of Cell Biology and Oncology, Istituto di Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro (Chieti), Italy. berrie@cmns.mnegri.it

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|December 2, 2000
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

GPR55 signalling promotes proliferation of pancreatic cancer cells and tumour growth in mice, and its inhibition increases effects of gemcitabine.

Oncogene·2018
Same author

The proline-rich tyrosine kinase Pyk2 regulates platelet integrin αIIbβ3 outside-in signaling.

Journal of thrombosis and haemostasis : JTH·2012
Same author

Targeting PDK1 in cancer.

Current medicinal chemistry·2011
Same author

The putative cannabinoid receptor GPR55 defines a novel autocrine loop in cancer cell proliferation.

Oncogene·2010
Same author

A novel inhibitor of the PI3K/Akt pathway based on the structure of inositol 1,3,4,5,6-pentakisphosphate.

British journal of cancer·2010
Same author

Role of class II phosphoinositide 3-kinase in cell signalling.

Biochemical Society transactions·2007

Extracellular inositol polyphosphates can inhibit cancer cell growth by blocking key signaling proteins from reaching the cell membrane. This discovery offers a new strategy for developing anticancer drugs targeting phosphoinositide 3-kinase pathways.

Area of Science:

  • Cellular signaling
  • Molecular biology
  • Oncology

Background:

  • Inositol polyphosphates and polyphosphoinositides are involved in various disease states.
  • Phosphoinositide 3-kinase (PI3K) and its 3-phosphorylated products are linked to oncogenesis.
  • PI3K downstream effectors regulate cellular signaling, cytoskeleton, and trafficking.

Purpose of the Study:

  • To investigate the intracellular effects of extracellular inositol polyphosphates.
  • To explore the therapeutic potential of inositol polyphosphate analogs in cancer treatment.
  • To establish a high-throughput screening method for identifying new anticancer drugs.

Main Methods:

  • Utilizing green fluorescent protein (GFP)-tagged PH domains to monitor protein localization.

Related Experiment Videos

  • Applying extracellular inositol polyphosphates in in vivo assay conditions.
  • Assessing the inhibition of membrane localization of specific protein domains.

    • Main Results:

    • Extracellular inositol polyphosphates selectively inhibit intracellular signaling pathways.
    • Inhibition of membrane localization of GFP-tagged PH domains was observed.
    • A correlation was found between inositol polyphosphate application and growth inhibition in assays.

    Conclusions:

    • Extracellular inositol polyphosphates demonstrate therapeutic potential in inhibiting cancer cell growth.
    • GFP-tagged polyphosphoinositide-binding domains can serve as tools for drug discovery.
    • Targeting membrane localization of specific protein domains is a promising strategy for anticancer drug development.