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A simple sequence repeat-based linkage map of barley.

L Ramsay1, M Macaulay, S degli Ivanissevich

  • 1Unit of Genomics, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland.

Genetics
|December 5, 2000
PubMed
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Researchers developed 568 new simple sequence repeat (SSR) markers for barley. These SSR markers provide a framework for future genetic mapping and offer an alternative to RFLP analysis in barley research.

Area of Science:

  • Genetics
  • Molecular Biology
  • Plant Science

Background:

  • Simple sequence repeats (SSRs) are valuable genetic markers.
  • Development of novel SSR markers is crucial for advancing barley genetics.

Purpose of the Study:

  • To develop and characterize a new set of SSR-based markers for barley.
  • To assess the informativeness and map these novel SSRs in barley.

Main Methods:

  • Developed 568 SSR markers from database sequences and genomic libraries.
  • Analyzed SSR informativeness across 16 barley cultivars.
  • Genetically mapped 242 SSRs in doubled-haploid populations from interspecific and intraspecific crosses.

Main Results:

  • SSR informativeness varied among cultivars, with no clear correlation to repeat characteristics.

Related Experiment Videos

  • 242 SSRs were successfully mapped, with 27 amplifying multiple loci.
  • Observed centromeric marker clustering, which was reduced in intraspecific crosses.

    • Conclusions:

    • The newly developed SSRs provide a robust framework for barley genetic mapping.
    • These markers offer a valuable alternative to RFLP for comparative mapping across populations.
    • The findings contribute to a better understanding of marker distribution and genetic effects in barley.