Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Plant stem cells: the only constant thing is change.

¹Institute of Cell and Molecular Biology, University of Edinburgh, Swann Building, EH9 3JR, Scotland, Edinburgh, UK. Peter.Doerner@ed.ac.uk

Current Biology : CB

|December 5, 2000

Summary

Arabidopsis WUSCHEL and CLAVATA genes form a negative feedback loop regulating stem cell fate in the shoot apical meristem. CLAVATA3 protein levels control this crucial developmental pathway.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Early primordium morphogenesis during lateral root initiation in Arabidopsis thaliana.

Planta·2002

Same author

Cell cycle controls: genome-wide analysis in Arabidopsis.

Current opinion in plant biology·2001

Same author

Plant meristems: a ménage à trois to end it all.

Current biology : CB·2001

Same author

Evidence for a role in growth and salt resistance of a plasma membrane H+-ATPase in the root endodermis.

The Plant journal : for cell and molecular biology·2001

Same author

Dynamic analyses of the expression of the HISTONE::YFP fusion protein in arabidopsis show that syncytial endosperm is divided in mitotic domains.

The Plant cell·2001

Same author

Root patterning: does auxin provide positional cues?

Current biology : CB·2000

Area of Science:

Plant biology
Developmental biology
Genetics

Background:

The shoot apical meristem (SAM) is crucial for plant development, maintaining stem cell populations.
WUSCHEL (WUS) and CLAVATA (CLV) genes are key regulators of SAM stem cell homeostasis.
Understanding the interplay between WUS and CLV is essential for deciphering stem cell fate control.

Purpose of the Study:

To elucidate the regulatory mechanisms coupling WUS and CLV functions in Arabidopsis SAM.
To investigate the role of CLAVATA3 protein abundance in modulating the WUS-CLV signaling pathway.

Main Methods:

Utilized genetic analysis in Arabidopsis thaliana.
Investigated gene expression patterns and protein localization.
Analyzed stem cell behavior in response to genetic perturbations.

Main Results:

Identified a negative feedback loop connecting WUS and CLV signaling pathways.
Demonstrated that WUS promotes stem cell proliferation, while CLV restricts it.
Showed that CLAVATA3 protein abundance directly limits the signaling output of this pathway.

Conclusions:

The WUS-CLV pathway establishes a critical balance for stem cell maintenance in the SAM.
CLAVATA3 acts as a key limiting factor, fine-tuning stem cell fate decisions.
This feedback mechanism ensures robust control over plant shoot development.

Related Experiment Videos