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Electromigration methods for amino acids, biogenic amines and aromatic amines.
1Department of Home Economics, Aichi-Gakusen University, Okazaki City, Japan.
Journal of Chromatography. B, Biomedical Sciences and Applications
|December 5, 2000
Summary
Capillary electrophoresis (CE) offers advanced analytical techniques for amino compounds. This review covers CE methods for detecting amino acids, amines, and their enantiomers, including single-cell and microchip applications.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Electromigration techniques in small-bore apparatus have rapidly advanced, yielding new analytical methods.
- Capillary electrophoresis (CE), a key electromigration technique, includes variations like capillary zone electrophoresis (CZE).
Purpose of the Study:
- To provide a concise overview of capillary electrophoresis (CE) methods for analyzing amino compounds.
- To summarize detection strategies and applications of CE for various amino compounds.
Main Methods:
- Review of published literature on CE techniques for amino compound analysis.
- Focus on detection methods for native and derivatized monoamines and polyamines.
- Description of current CE methods and their applications.
Main Results:
- CE is effective for detecting amino acids, biogenic amines, aromatic amines, and heteroaromatic amines.
- CE can differentiate enantiomers of these compounds.
- New CE methods are emerging for single-cell analysis and microchip applications.
Conclusions:
- Capillary electrophoresis provides versatile and sensitive methods for amino compound analysis.
- CE applications are expanding into complex biological samples like single cells.
- Microchip CE offers potential for miniaturized and high-throughput analysis.