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Updated: Jul 15, 2026
13:35
A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
Forensic Electrochemistry: A Dual-Mode Strategy for Rapid and Selective Detection of Catecholamine Compounds
Larissa M A Melo1,2, Elena Bernalte1, Robert D Crapnell1
1Faculty of Science and Engineering, Dalton Building, Manchester Metropolitan University, Chester Street, ManchesterM1 5GD, United Kingdom.
Analytical Chemistry
|July 14, 2026
Summary
A new colorimetric-electrochemical method accurately detects epinephrine (EPN) in forensic samples. This sensitive technique offers reliable screening for EPN and related catecholamines in biological matrices.
Area of Science:
- Analytical Chemistry
- Forensic Science
Background:
- Catecholamines, like epinephrine (EPN), are crucial biomarkers.
- Accurate detection of EPN in forensic samples is challenging.
Purpose of the Study:
- Develop a novel colorimetric-electrochemical strategy for selective catecholamine detection.
- Apply the method for epinephrine analysis in forensic samples.
Main Methods:
- Integrated colorimetric Melo Test with a two-stage electrochemical protocol.
- Utilized screen-printed graphite electrodes (SPE-Gr) and square-wave voltammetry.
- Analyzed three responses: EPN oxidation (O1), colorimetric reaction, and product oxidation (P1).
Main Results:
- Achieved good linearity (6-60 μg L-1) and low limits of detection (1.5-2.3 μg L-1).
- Demonstrated high reproducibility (RSD <5%) and selectivity in biological matrices.
- Successfully discriminated between different catecholamine structures.
Conclusions:
- The developed method is a reliable tool for epinephrine screening in forensic toxicology.
- The approach shows potential for analyzing other catecholamine compounds.
- Offers a sensitive and selective detection strategy for complex biological samples.
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