A quantitative and qualitative high performance liquid chromatographic determination of acetaminophen and five of its...

An accurate HPLC determination of acetaminophen in tablet dosage form is reported, together with an effective separation of five of its para-substituted derivatives using an isocratic reversed-phase system. The column consisted of an octadecylsilane 10 microm stationary phase. The mobile phase was a mixture of acetonitrile and water (7:3). The retention times for acetaminophen, O-acetylacetaminophen, O-ethylacetaminophen, O-(2-nitrobenzenesulphonyl)acetaminophen, O-benzylacetaminophen and O-(3,5-dinitrobenzoyl)acetaminophen were 2.83, 3.25, 3.56. 3.78, 4.37 and 6.03 min, respectively.