Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Clinical practice--improving instruction and evaluating performance.

L T Gurley

    Radiologic Technology
    |January 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Pro & con: staff development vs in-service training. In-service education.

    Applied radiology·1987
    Same author

    New opportunities in containing education.

    Applied radiology·1981
    Same author

    Jerman Memorial Lecture. Computer reconstruction of radiographic images.

    Radiologic technology·1980
    Same author

    Criteria-referenced approach to clinical practicum.

    Radiologic technology·1975
    Same author

    A practical technique for baseline mammography.

    Radiologic technology·1972
    Same author

    Criteria for the evaluation of an inservice education program.

    Radiologic technology·1971
    Same journal

    An Intentional and Ethical Integration of AI in Medical Imaging.

    Radiologic technology·2026
    Same journal

    Benefits of Integrating AI Into Computer-Aided Detection Systems.

    Radiologic technology·2026
    Same journal

    Using Artificial Intelligence to Enhance Analysis of Chest Computed Tomography.

    Radiologic technology·2026
    Same journal

    A Practice-Aligned Approach to Integrating AI in Radiation Sciences Education.

    Radiologic technology·2026
    Same journal

    Site Visitors: The Unsung Heroes of the Accreditation Process.

    Radiologic technology·2026
    Same journal

    Extended Reality Innovations in Medical Imaging Education.

    Radiologic technology·2026
    See all related articles

    Researchers developed ouabain-resistant (OR) HeLa cells that tolerate high drug concentrations. These OR cells exhibit altered sodium pump affinities, explaining their resistance to cardiac glycosides and other active transport inhibitors.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Pharmacology

    Background:

    • Cardiac glycosides like ouabain are toxic to HeLa cells at low concentrations.
    • Active transport of sodium (Na+) across the plasma membrane is crucial for cell volume regulation.
    • Developing drug-resistant cell lines is essential for understanding drug mechanisms.

    Purpose of the Study:

    • To investigate the mechanisms underlying ouabain resistance in HeLa cells.
    • To compare the sensitivity of ouabain-sensitive (OS) and ouabain-resistant (OR) cells to various cardiac glycosides and related compounds.
    • To elucidate the role of sodium pump affinity in differential drug sensitivity.

    Main Methods:

    • Cultivating HeLa cells with increasing ouabain concentrations to generate an OR cell population.

    Related Experiment Videos

  • Assessing inhibition of cell volume regulation under hypotonic shock to measure active Na+ transport.
  • Determining dose-response curves for ouabain and other inhibitors in both OS and OR cells.

    • Main Results:

    • OR cells demonstrated resistance to ouabain, growing in concentrations up to 10-4 M, unlike sensitive OS cells.
    • Other cardiac glycosides and aglycones showed similar inhibitory effects on OS cells but were ineffective against OR cells at tested concentrations.
    • Coumingine sensitivity differed between OS and OR cells, with OR cells being less sensitive.
    • Ouabain binding experiments and reversal rate measurements supported altered sodium pump affinities in OR cells.

    Conclusions:

    • The acquired resistance in OR cells is attributed to altered affinities of their sodium pumps for active transport inhibitors.
    • Differential drug sensitivities highlight the specific interactions between cardiac glycosides and the sodium pump.
    • This study provides insights into the molecular basis of drug resistance and sodium pump function.