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Acidic endomembrane organelles are required for mouse postimplantation development
G Sun-Wada1, Y Murata, A Yamamoto
1Division of Biological Sciences, The Institute of Scientific and Industrial Research, Osaka University, Toyonaka, Osaka, Japan.
Developmental Biology
|December 9, 2000
Summary
Acidic compartments created by vacuolar-type H(+)-ATPase (V-ATPase) are vital for mouse embryo development post-implantation. Loss of the V-ATPase c subunit impairs cell proliferation and organelle function, leading to developmental failure.
Area of Science:
- Developmental Biology
- Cell Biology
- Molecular Biology
Background:
- Vacuolar-type H(+)-ATPase (V-ATPase) is crucial for proton transport in eukaryotic cells.
- Acidic compartments are essential for various cellular processes, including endocytosis and organelle function.
Purpose of the Study:
- To investigate the role of V-ATPase-generated acidic compartments in mouse preimplantation and postimplantation development.
- To determine the consequences of lacking the V-ATPase 16-kDa proteolipid (c subunit) on embryonic development.
Main Methods:
- Analysis of V-ATPase acidic compartments in mouse embryos from the one-cell stage to blastocyst.
- Generation and in vitro/in vivo analysis of V-ATPase c subunit knockout (PL16(-/-)) mouse embryos.
- Assessment of cellular processes like endocytosis, organellar acidification, and Golgi complex morphology in PL16(-/-) cells.
Main Results:
- Acidic compartments are present from the earliest stages of mouse embryonic development and show polarized distribution at the blastocyst stage.
- PL16(-/-) embryos implant but fail to develop further, exhibiting severe defects in embryonic and extraembryonic tissues.
- PL16(-/-) blastocysts show impaired endocytosis, reduced organellar acidification, and Golgi abnormalities, leading to slow inner cell mass growth and cell death in vitro.
Conclusions:
- Acidic compartments generated by V-ATPase are indispensable for postimplantation development in mice.
- The V-ATPase c subunit is critical for maintaining cellular homeostasis and supporting rapid proliferation required after implantation.