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Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
Published on: December 26, 2011
A soybean G2 glycinin allergen. 1. Identification and characterization
R M Helm1, G Cockrell, C Connaughton
1Department of Pediatrics, University of Arkansas for Medical Sciences, Arkansas Children's Nutrition Center, Little Rock, AR 72202-3591, USA.
International Archives of Allergy and Immunology
|December 12, 2000
Summary
Soybean allergy is linked to specific proteins. Researchers identified a 22-kD soybean allergen, a glycinin family member, responsible for allergic reactions in sensitive individuals.
Area of Science:
- Food allergy research
- Protein biochemistry
- Immunology
Background:
- Soybean extracts contain multiple allergens.
- Specific IgE in allergic individuals binds to a ~21 kD soybean protein.
- Unique soybean proteins may cause allergic reactions.
Purpose of the Study:
- Identify unique soybean proteins binding specific IgE.
- Characterize the soybean allergen using physicochemical methods and IgE binding.
Main Methods:
- Two-dimensional and preparative SDS-PAGE/IgE immunoblot analysis.
- N-terminal sequence analysis of the identified protein.
Main Results:
- A 22-kD soybean allergen was identified.
- The allergen belongs to the G2 glycinin soybean protein family.
- IgE antibodies reacted with basic chains of glycinin family members.
Conclusions:
- Glycinin subunits, prevalent soybean storage proteins, are major allergens.
- Allergenicity is associated with the glycinin protein family in soybeans.
