Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Membrane targeting: what a difference a G makes.

P J Cullen1, P Chardin

  • 1Department of Biochemistry, University of Bristol, BS8 1TD,., Bristol, UK. Pete.Cullin@bris.ac.uk

Current Biology : CB
|December 15, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biomolecules as Model Indicators of <i>In Vitro</i> and <i>In Vivo</i> Cold Plasma Safety.

Frontiers in physics·2023
Same author

Inactivation efficacy of atmospheric air plasma and airborne acoustic ultrasound against bacterial biofilms.

Scientific reports·2021
Same author

Developments and Challenges in Online NIR Spectroscopy for Meat Processing.

Comprehensive reviews in food science and food safety·2020
Same author

Impact of cold plasma processing on major peanut allergens.

Scientific reports·2020
Same author

Perspectives from CO+RE: How COVID-19 changed our food systems and food security paradigms.

Current research in food science·2020
Same author

The Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using <i>Listeria monocytogenes</i> Knockout Mutants.

Frontiers in microbiology·2020
Same journal

Hunting ecology predicts eye arrangements in the modular visual system of spiders.

Current biology : CB·2026
Same journal

Sub-second fluctuations between top-down and bottom-up modes distinguish diverse human brain states.

Current biology : CB·2026
Same journal

Queen bees offload pesticide burden to eggs when social buffering is overwhelmed.

Current biology : CB·2026
Same journal

Pitch selectivity in ferret auditory cortex.

Current biology : CB·2026
Same journal

A cell size-dependent competition between geometry and polarity governs nuclear and spindle positioning in early embryos.

Current biology : CB·2026
Same journal

Trophic cascades drive sustainability in the agricultural heritage rice-fish coculture system.

Current biology : CB·2026
See all related articles

Pleckstrin homology domains target proteins to membranes by binding polyphosphoinositides. Recent findings reveal new insights into how these domains bind 3-phosphoinositides, uncovering surprising details.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Pleckstrin homology (PH) domains are crucial protein modules.
  • They mediate protein localization to cellular membranes.
  • This localization is primarily achieved through interactions with phosphoinositides.

Purpose of the Study:

  • To elucidate the binding mechanisms of PH domains to 3-phosphoinositides.
  • To highlight recent advancements and unexpected findings in this area.
  • To deepen the understanding of protein-membrane interactions mediated by PH domains.

Main Methods:

  • Structural biology techniques (e.g., X-ray crystallography, NMR spectroscopy).
  • Biochemical assays to measure binding affinities and specificities.

Related Experiment Videos

  • Cellular imaging to observe protein localization in vivo.

    • Main Results:

    • Detailed structural and biochemical characterization of PH domain interactions with specific 3-phosphoinositides.
    • Identification of key amino acid residues and conformational changes involved in binding.
    • Observation of previously unrecognized binding modes or specificities.

    Conclusions:

    • PH domains exhibit diverse and sometimes unexpected mechanisms for binding 3-phosphoinositides.
    • These findings refine our understanding of phosphoinositide-mediated protein recruitment.
    • The study provides a foundation for further research into signaling pathways involving PH domains.