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Updated: Aug 9, 2026

Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
Connecting signaling and cell cycle progression in growth factor-stimulated cells
1Schepens Eye Research Institute, 20 Staniford Street, Boston, Massachusetts, MA 02114, USA.
Abstract:
A widely used model system to investigate cell proliferation is stimulation of serum-arrested cells with growth factors. Recent data suggest that there are two waves of growth factor-dependent signaling events required for a proliferative response. One is an acute burst of signaling, which occurs immediately after growth factor stimulation and lasts for 30 - 60 min. The other occurs in a different time frame (8 - 12 h post stimulation), and involves activation of cyclin dependent kinases (Cdks). In addition to a general overview of growth factor-dependent signaling, we present our 'two wave' hypothesis for how signaling and cell cycle progression are linked.
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