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Updated: Oct 10, 2026

Single Particle Cryo-Electron Microscopy: From Sample to Structure
Published on: May 29, 2021
Macromolecular electron microscopy in the era of structural genomics
1Dept of Molecular Structural Biology, Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, D-82152 Martinsried, Germany. baumeist@biochem.mpg.de
Abstract:
Macromolecular machines carry out many cellular functions. Cryo-electron microscopy (cryo-EM) is emerging as a powerful method for studying the structure, assembly and dynamics of such macromolecules, and their interactions with substrates. With resolutions still improving, 'single-particle' analyses are already depicting secondary structure. Moreover, cryo-EM can be combined in several ways with X-ray diffraction to enhance the resolution of cryo-EM and the applicability of crystallography. Electron tomography holds promise for visualizing machines at work inside cells.
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