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A novel RING finger-B box-coiled-coil protein, GERP.

S R Vincent1, D A Kwasnicka, P Fretier

  • 1Division of Neuroscience, Brain Research Centre, Vancouver, British Columbia, V6T 1Z3, Canada.

Biochemical and Biophysical Research Communications
|December 19, 2000
PubMed
Summary
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Researchers identified a novel glioblastoma expressed RING finger protein (GERP) in various human tissues and tumors. Its location on chromosome 10q24.3 suggests GERP may function as a tumor suppressor gene in gliomas.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • The RING finger domain is crucial for protein regulation in cells.
  • Novel RING finger proteins are sought using techniques like PCR and expressed sequence tags (ESTs).

Purpose of the Study:

  • To identify and characterize a novel RING finger protein expressed in human tissues and tumors.
  • To investigate the potential role of this protein in cancer, particularly gliomas.

Main Methods:

  • Utilized polymerase chain reaction (PCR) with primers from expressed sequence tags (ESTs) to find new RING finger proteins.
  • Cloned and sequenced a novel cDNA encoding a RING finger protein.
  • Determined gene expression patterns in various normal tissues and tumors.
  • Mapped the gene's location to chromosome 10q24.3.

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Main Results:

  • A novel RING finger protein, glioblastoma expressed RING finger protein (GERP), was identified.
  • GERP is expressed in brain, lung, breast, placenta, kidney, muscle, and germinal center B cells.
  • The gene is expressed in tumors like anaplastic oligodendroglioma and maps to 10q24.3, a region associated with tumor suppressor genes.
  • GERP possesses an N-terminal RING finger, two B-boxes, and a coiled-coil, classifying it in the RBCC subfamily.

Conclusions:

  • GERP is a novel RBCC subfamily RING finger protein identified through PCR-based screening.
  • Its expression profile and chromosomal location suggest GERP may be a novel tumor suppressor gene.
  • GERP could play a significant role in the development of gliomas and other cancers.