Jove
Visualize
Contact Us

Related Experiment Videos

Overlapping expression of zebrafish T-brain-1 and eomesodermin during forebrain development.

M Mione1, S Shanmugalingam, D Kimelman

  • 1Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK. m.mione@ucl.ac.uk

Mechanisms of Development
|December 19, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The LAST word on safety: an iterative quality improvement study of local anaesthetic documentation.

Anaesthesia reports·2026
Same author

Australian Registered Nurse's' Awareness of Key Issues, Ambivalence and Education Related to the Health of People With Intellectual Disability and/or Autism.

Journal of applied research in intellectual disabilities : JARID·2025
Same author

Is the practice of case-tracking a substitute for traditional feedback?

Medical teacher·2024
Same author

Giant parathyroid lipoadenoma case report: A parathyroid phenomenon.

International journal of surgery case reports·2024
Same author

The direct impact of pegvisomant on osteoblast functions and bone development.

Journal of endocrinological investigation·2023
Same author

Correction to: A synopsis of global frontiers in fertility preservation.

Journal of assisted reproduction and genetics·2022
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Zebrafish T-brain-1 (tbr1) and eomesodermin (eom) are T-box genes crucial for embryonic cell fate. Their expression patterns in the developing brain reveal differential roles in neuroectodermal fate determination.

Area of Science:

  • Developmental Biology
  • Neuroscience
  • Genetics

Background:

  • T-box transcription factors regulate embryonic cell fate.
  • Two T-box genes, tbr1 and eomesodermin (eom), are expressed in the vertebrate telencephalon.

Purpose of the Study:

  • To clone zebrafish tbr1 and eom.
  • To investigate their expression patterns during zebrafish embryogenesis and larval development.
  • To understand their roles in neuroectodermal fate determination.

Main Methods:

  • Cloning of zebrafish tbr1 and eom.
  • Whole-mount in situ hybridization to analyze gene expression patterns.
  • Microscopic examination of embryonic and larval zebrafish brains.

Main Results:

Related Experiment Videos

  • Eom is expressed early in the presumptive telencephalon, expanding to the dorsal telencephalon by the 22 somite stage.
  • Tbr1 expression begins later (18 somite stage) within a subset of eom-expressing cells.
  • By 24 hours post-fertilization, tbr1 and eom show overlapping expression in the dorsal telencephalon, with tbr1 in postmitotic cells and eom in proliferative cells. Both genes are also found in the diencephalon.

Conclusions:

  • Zebrafish tbr1 and eom are differentially expressed in distinct cell populations of the developing brain.
  • Their distinct expression patterns suggest specific roles in the development of the telencephalon and diencephalon.
  • These findings provide a foundation for genetic studies on neuroectodermal fate determination in zebrafish.