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Thyroid stimulating hormone increases angiogenic growth factor expression in rat thyrocytes
1(Division of Medical Sciences, Queen Elizabeth Hospital, Edgbaston, Birmingham, UK).
Clinical Otolaryngology and Allied Sciences
|December 21, 2000
Summary
Thyroid stimulating hormone (TSH) increases expression of fibroblast growth factor-2 (FGF-2), its receptor (FGFR-1), and Tie-2 in rat thyroid cells. These findings suggest a role for TSH in goitrogenesis-related angiogenesis.
Area of Science:
- Endocrinology
- Molecular Biology
- Cell Biology
Background:
- Goitrogenesis involves thyroid stimulating hormone (TSH) receptor hyperstimulation.
- Angiogenic factors like fibroblast growth factor-2 (FGF-2), FGFR-1, and Tie-2 are elevated in goiters.
Purpose of the Study:
- Investigate the role of TSH in regulating FGF-2, FGFR-1, and Tie-2 expression in rat thyroid cells (FRTL-5).
Main Methods:
- FRTL-5 cells were cultured and exposed to varying TSH concentrations.
- Protein expression of FGF-2, FGFR-1, and Tie-2 was analyzed using Western blotting.
Main Results:
- TSH elevated FGF-2 expression, peaking at 0.3 mU/ml.
- TSH dose-dependently increased FGFR-1 fragment expression.
- TSH upregulated full-length Tie-2 receptor expression on FRTL-5 cells, mediated by cAMP.
Conclusions:
- Elevated TSH increases FGF-2, FGFR-1, and Tie-2 in rat thyroid cells.
- These TSH-induced growth factors may drive angiogenesis in goitrogenesis.
- Targeting these factors could offer new therapeutic strategies for goiter.