Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A Particle-Level Model of Irreversible Protein Adsorption with a Postadsorption Transition

Van Tassel PR1, Guemouri, Ramsden

  • 1Department of Chemical Engineering and Materials Science, Wayne State University, Detroit, Michigan, 48202

Journal of Colloid and Interface Science
|October 30, 1998
PubMed
Summary

This study introduces a particle-level kinetic model for protein adsorption, considering surface blockage and conformational changes. The model accurately predicts protein density evolution on surfaces, validated by fibronectin adsorption experiments.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Video casebook: medicine-The toy boy and the burgundy Car

BMJ (Clinical research ed.)·2001
Same author

14. FDG CDET (2D Dual-Head Coincidence Gamma Camera) in the Primary Staging of Oesophageal Cancer. Histopathological Correlation.

Clinical positron imaging : official journal of the Institute for Clinical P.E.T·2001
Same author

15. Sensitivity of FDG CDET (2D Dual-Head Coincidence Gamma Camera) for the Detection of Occult or Doubtful Recurrences of Colorectal Cancer. Histopathological Correlation.

Clinical positron imaging : official journal of the Institute for Clinical P.E.T·2001
Same author

32. Detection of Recurrence of Ovarian Cancer using

Clinical positron imaging : official journal of the Institute for Clinical P.E.T·2001
Same author

Phase diagram of an ising model with long-range frustrating interactions: A theoretical analysis

Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics·2001
Same author

Thyroid stimulating hormone increases angiogenic growth factor expression in rat thyrocytes

Clinical otolaryngology and allied sciences·2000

Area of Science:

  • Biophysics
  • Surface Science
  • Chemical Engineering

Background:

  • Accurate modeling of protein adsorption kinetics is crucial for applications like biosensor design and understanding biological responses.
  • Existing models often lack detailed consideration of geometric surface blockage and post-adsorption protein behavior.

Purpose of the Study:

  • To develop a particle-level kinetic model for protein adsorption that incorporates geometric surface blockage and conformational changes.
  • To provide a framework for predicting the time evolution of adsorbed protein density on solid surfaces.

Main Methods:

  • Developed a kinetic model treating proteins as disk-shaped particles undergoing irreversible adsorption and spreading.
  • Formulated a set of equations to describe the temporal changes in adsorbed protein density.

Related Experiment Videos

  • Validated the model using experimental data of fibronectin adsorption onto silica-titania.

    • Main Results:

    • The model successfully accounts for surface blockage and post-adsorption spreading, influencing the final protein density.
    • Predictions from the model show good agreement with experimental data obtained via optical waveguide lightmode spectroscopy (OWLS).

    Conclusions:

    • The developed kinetic model offers a robust approach for simulating protein adsorption, including geometric constraints and protein rearrangement.
    • This model has broad applicability for analyzing experimental protein adsorption data and informing the design of surface-based technologies.