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Control of T cell development in vivo by subdomains within the IL-7 receptor alpha-chain cytoplasmic tail

B O Porter1, P Scibelli, T R Malek

  • 1Department of Microbiology and Immunology, University of Miami School of Medicine, Miami, FL 33101, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|December 21, 2000
PubMed

IL-7/IL-7R signaling functions in both growth and differentiation during T cell development. In this study, we examined the extent these activities were controlled by signaling associated with distinct IL-7R alpha cytoplasmic domains by transgenic expression of wild-type or cytoplasmic deletion mutants of IL-7R alpha in the thymi of IL-7R alpha(-/-) mice. We show an essential requirement for the tyrosine-containing carboxyl-terminal T domain in restoring thymic cellularity, pro-/pre-T cell progression, and survival. In contrast, the functional differentiation of TCR alpha beta cells and the development of TCR gamma delta cells are partially independent of the T domain. Thus, separate cytoplasmic domains of the IL-7R alpha chain differentially control distinct functions during T cell development, whereas normal IL-7R-dependent thymic development requires the integrated activity of all these domains.

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