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BodyMap incorporated PCR-based expression profiling data and a gene ranking system
J Sese1, H Nikaidou, S Kawamoto
1Department of Complexity Science and Engineering, Graduate School of Frontier Science, University of Tokyo, 7-3-1 Hongo, Bunkyo Word, Tokyo 113-0033, Japan.
Nucleic Acids Research
|January 11, 2000
Summary
BodyMap is a gene expression database featuring over 300,000 human and mouse gene sequences. Recent updates include precise anatomical expression patterns for over half of human genes using introduced amplified fragment length polymorphism (iAFLP) analysis.
Area of Science:
- Genomics
- Bioinformatics
- Gene Expression Analysis
Background:
- BodyMap is a comprehensive gene expression database.
- It is based on site-directed 3'-expressed sequence tags.
- Contains over 300,000 sequences from human and mouse tissues.
Purpose of the Study:
- To enhance the BodyMap database with precise anatomical gene expression patterns.
- To incorporate high-throughput expression profiling data.
- To develop a gene ranking system for users.
Main Methods:
- Site-directed 3'-expressed sequence tags generation.
- Introduced amplified fragment length polymorphism (iAFLP) for high-throughput expression profiling.
- Development of a novel gene ranking system.
Main Results:
- BodyMap now includes precise anatomical expression patterns for over half of human gene entries.
- Incorporated iAFLP data covers relative contents of over 12,000 transcripts across 30 tissue RNAs.
- A new gene ranking system allows users to find genes with specific expression patterns.
Conclusions:
- BodyMap provides valuable, detailed gene expression data for human and mouse.
- The integration of iAFLP enhances the understanding of gene expression patterns.
- The database supports data transfer and WWW-accessible analysis.