Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cochlear microinjection and its effects upon auditory function in the guinea pig.

S T Kho1, R M Pettis, A N Mhatre

  • 1Department of Otolaryngology--Head and Neck Surgery, University of California San Francisco, 94117, USA.

European Archives of Oto-Rhino-Laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
|December 29, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Small molecule delivery across a perforated artificial membrane by thermoreversible hydrogel poloxamer 407.

Colloids and surfaces. B, Biointerfaces·2019
Same author

Utility of electronystagmography in the prediction of post-operative outcome following cochlear implantation.

The Journal of laryngology and otology·2015
Same author

Scanning electrochemical microscopy as a novel proximity sensor for atraumatic cochlear implant insertion.

IEEE transactions on bio-medical engineering·2014
Same author

The cochlear-carotid interval: anatomic variation and potential clinical implications.

AJNR. American journal of neuroradiology·2006
Same author

SDHB, SDHC, and SDHD mutation screen in sporadic and familial head and neck paragangliomas.

Clinical genetics·2004
Same author

Refinement of the DFNA4 locus to a 1.44 Mb region in 19q13.33.

Journal of molecular medicine (Berlin, Germany)·2004

Microinjection via the round window membrane for gene therapy in guinea pigs did not cause permanent hearing loss. Temporary auditory function changes were observed but fully recovered within four weeks.

Area of Science:

  • Oto-rhino-laryngology
  • Neuroscience
  • Gene Therapy

Background:

  • Microinjection via the round window membrane is a potential method for intracochlear gene transfer.
  • This technique breaches the round window membrane, necessitating evaluation for hearing loss.
  • Assessing the safety of this delivery method is crucial for future therapeutic applications.

Purpose of the Study:

  • To evaluate the impact of saline microinjection through the round window membrane on auditory function in guinea pigs.
  • To determine if microinjection causes permanent or reversible hearing loss.

Main Methods:

  • Healthy guinea pigs underwent baseline auditory brainstem response (ABR) threshold testing.
  • Saline was microinjected through the round window membrane.

Related Experiment Videos

  • Auditory function was reassessed using ABR at one week and, if necessary, at four weeks post-injection.

    • Main Results:

    • Postoperative ABR thresholds remained unchanged from baseline in 71% of animals.
    • Two animals experienced temporary 20-25 dB increases in ABR thresholds, which recovered to baseline levels by four weeks.
    • The mean ABR threshold difference between pre- and post-injection was not statistically significant (P = 0.707).

    Conclusions:

    • Saline microinjection through the round window membrane did not induce permanent hearing loss in the tested guinea pigs.
    • Any hearing impairment resulting from microinjection appears to be transient and reversible.
    • This preliminary study supports the potential safety of round window membrane microinjection for intracochlear gene delivery.