[Human DNA polymorphism of HaeIII system in Chinese oriental, Han population]

DNA typing by restriction fragment length polymorphism (RFLP) analysis was an extremely important technique used in forensic science identification, paternity test and gene diagnosis. While RFLP testing was a highly informative method, it traditionally had several disadvantages. It was time consuming and involved in working with radioactive phosphorus. A chemiluminescent detection of RFLP technique that was faster and safer than isotopic detection. The chemiluminescent system had a good sensitivity of detecting 3-25 ng sample DNA. The development of direct alkaline phosphatase (AP) conjugated probes and improved chemiluminescent substrates provided a non-isotopic detection method that equaled or surpassed 32P detection systems. Population genetic studies were performed using direct alkaline phosphatase (AP) conjugated oligonucleotide probes (AL1874, YNH24, TBQ7, VI) that recognized four hypervariable number of tandem repeats regions (D2S92, D2S44, D10S28, D17S79 loci) respectively in the human genome. DNA from approximately 480 unrelated individuals, subdivided into oriental group were digested with HaeIII enzyme and were successively hybridized to each DNA probe. The number of distinct DNA fragments identified for each of these regions varies from 37 to more than 78. An allele frequency distribution was determined for each locus with the computer and digitizer. The results showed significant differences between different races (American-blacks, Caucasians, and Orientals), in the pattern of distributions as well as in the relative frequency of the most common alleles of D2S92, D2S44, D10S28, and D17S79. The results showed that the analysis of these loci had useful applications in genetics and in forensic identity.