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Host-phage interaction on Agrobacterium tumefaciens. IV. Phage-directed protein synthesis
Journal of Virology
|February 1, 1975
Summary
Agrobacterium tumefaciens infected with phage LV-1 showed temporal protein synthesis. Early proteins shut down host synthesis, followed by class II and late class III proteins, including phage endolysin.
Area of Science:
- Microbiology
- Molecular Biology
- Virology
Background:
- Bacteriophages are viruses that infect bacteria.
- Bacteriophage infection triggers changes in host cell protein synthesis.
Purpose of the Study:
- To investigate the temporal sequence of protein synthesis in Agrobacterium tumefaciens after infection with phage LV-1.
Main Methods:
- Gel electrophoresis and autoradiography were employed to analyze protein synthesis.
- Temporal protein expression was monitored post-phage infection.
Main Results:
- Three distinct classes of proteins were identified: early (Class I), intermediate (Class II), and late (Class III).
- Class I proteins include a factor that inhibits host protein synthesis.
- Class III proteins, appearing at 45 minutes, include phage endolysin and five other proteins.
Conclusions:
- Phage LV-1 infection orchestrates a specific temporal program of protein synthesis in Agrobacterium tumefaciens.
- The identified protein classes play distinct roles in the phage replication cycle.