Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-density microarray-mediated gene expression profiling of Escherichia coli.

Y Wei1, J M Lee, C Richmond

  • 1Central Research and Development, DuPont Company, Wilmington, Delaware 19880-0173, USA.

Journal of Bacteriology
|January 3, 2001
PubMed
Summary

This study developed a high-density array for analyzing Escherichia coli gene expression. Researchers quantified mRNA levels, revealing distinct patterns across growth phases and media conditions for improved E. coli physiology understanding.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Determining the sources of nutrient flux to water in headwater catchments: Examining the speciation balance to inform the targeting of mitigation measures.

The Science of the total environment·2018
Same author

Root Ideotype Influences Nitrogen Transport and Assimilation in Maize.

Frontiers in plant science·2018
Same author

A prospective observational study of the association between cabin and outside air temperature, and patient temperature gradient during helicopter transport in New South Wales.

Anaesthesia and intensive care·2016
Same author

High-sensitivity ring-down evanescent-wave sensing in fiber resonators.

Optics letters·2014
Same author

The Health Insurance Plan of Greater New York.

Public health nursing·2010
Same author

A revision of Phoreiobothrium (Tetraphyllidea: Onchobothriidae) with descriptions of five new species.

The Journal of parasitology·2006

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genomics

Background:

  • High-density arrays are crucial for comprehensive gene expression analysis.
  • Understanding Escherichia coli gene regulation is vital for various biological applications.

Purpose of the Study:

  • To develop and validate a high-density array for analyzing the transcriptome of Escherichia coli.
  • To quantify the steady-state mRNA levels of nearly all E. coli open reading frames.
  • To investigate differential gene expression under varying growth conditions.

Main Methods:

  • Amplification and arraying of 4,290 Escherichia coli open reading frames on glass slides.
  • Optimization of RNA isolation, cDNA synthesis with fluorescent labeling, and DNA-DNA hybridization.
  • Quantification of hybrid signals and normalization using a genomic DNA probe for accurate transcript abundance estimation.

Related Experiment Videos

Main Results:

  • Established robust protocols for gene expression profiling of E. coli.
  • Observed a 30-fold increase in lacZ, lacY, and lacA transcripts upon isopropyl-beta-D-thiogalactopyranoside (IPTG) induction, with minimal changes in other transcripts.
  • Catalogued distinct RNA expression patterns between exponential and transitional growth phases and between minimal and rich media.

Conclusions:

  • The developed array provides a quantitative snapshot of the E. coli transcriptome.
  • The findings offer insights into E. coli gene regulation and physiology under different conditions.
  • This method enables systematic enumeration and comparison of gene expression data with existing physiological knowledge.