Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

X-ray Crystallography02:18

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...
Determination of Crystal Structures01:29

Determination of Crystal Structures

In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Analysis of Particles at Low Accelerating Voltages (≤ 10 kV) With Energy Dispersive X-Ray Spectroscopy (EDS).

Journal of research of the National Institute of Standards and Technology·2016
Same author

Comparison of channeling contrast between ion and electron images.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2013
Same author

Controlled fabrication of nanopores using a direct focused ion beam approach with back face particle detection.

Nanotechnology·2011
Same author

Effects of low versus physiologic plasma progesterone concentrations on ovarian follicular development and fertility in beef cattle.

Theriogenology·2009
Same author

Effects of progesterone presynchronization and eCG on pregnancy rates to GnRH-based, timed-AI in beef cattle.

Theriogenology·2008
Same author

Lower pregnancy losses in lactating dairy cows fed a diet enriched in alpha-linolenic acid.

Journal of dairy science·2006

Related Experiment Video

Updated: Jul 28, 2026

Characterization of Ultra-fine Grained and Nanocrystalline Materials Using Transmission Kikuchi Diffraction
09:13

Characterization of Ultra-fine Grained and Nanocrystalline Materials Using Transmission Kikuchi Diffraction

Published on: April 1, 2017

Phase identification of individual crystalline particles by electron backscatter diffraction.

J A Small1, J R Michael

  • 1National Institutes of Standards and Technology, Gaithersburg, MD 20899-8371, USA. john.small@nist.gov

Journal of Microscopy
|January 3, 2001
PubMed
Summary

This study demonstrates the successful application of an electron backscatter diffraction (EBSD) system for phase identification in individual micro- and sub-micrometre particles, expanding its utility beyond traditional bulk samples.

More Related Videos

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene
08:44

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
10:12

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

Published on: June 19, 2018

Related Experiment Videos

Last Updated: Jul 28, 2026

Characterization of Ultra-fine Grained and Nanocrystalline Materials Using Transmission Kikuchi Diffraction
09:13

Characterization of Ultra-fine Grained and Nanocrystalline Materials Using Transmission Kikuchi Diffraction

Published on: April 1, 2017

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene
08:44

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
10:12

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

Published on: June 19, 2018

Area of Science:

  • Materials Science
  • Crystallography
  • Analytical Chemistry

Background:

  • A novel electron backscatter diffraction (EBSD) system utilizing a high-resolution CCD camera and phosphor screen has been developed.
  • This system integrates crystallographic data from EBSD patterns with elemental data from X-ray spectrometry for phase identification.
  • Current applications are predominantly limited to polished, bulk samples.

Purpose of the Study:

  • To investigate the applicability of the advanced EBSD system for phase identification of individual micro- and sub-micrometre particles.
  • To extend the analytical capabilities of EBSD beyond conventional flat surfaces.

Main Methods:

  • Utilized an EBSD system with a 1024 x 1024 CCD camera and phosphor screen.
  • Combined EBSD pattern analysis with energy or wavelength dispersive X-ray spectrometry.
  • Employed a commercial diffraction database for crystalline phase identification.

Main Results:

  • Successfully applied the EBSD system to analyze individual micrometre and sub-micrometre particles.
  • Demonstrated effective phase identification for particulate samples, not just flat surfaces.
  • Validated the system's capability to analyze smaller, individual sample entities.

Conclusions:

  • The EBSD system is effective for phase identification analysis of individual micro- and sub-micrometre particles.
  • This research expands the application scope of EBSD technology to nanoscale and microscale particulate analysis.
  • The findings pave the way for more versatile material characterization using EBSD.