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Modified salting-out method for DNA isolation from newborn cord blood nucleated cells
N I Noguera1, C E Tallano, I M Bragós
1Cátedra y Servicio de Hematología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Hospital Provincial del Centenario, Rosario, Santa Fe, Argentina. nnofuwe@fbioyf.unr.edu.ar
Abstract:
The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the beta-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a considerable number of cord blood samples. By introducing a simple phenol/chloroform step, before ethanol precipitation of the nucleic acid, to certain samples, we were able to eliminate or substantially reduce the presence of inhibitors to PCR without having to re-extract the samples.