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Related Experiment Videos

CCR7 expression and memory T cell diversity in humans.

J J Campbell1, K E Murphy, E J Kunkel

  • 1Joint Program in Transfusion Medicine, Children's Hospital, and Harvard Medical School, Department of Pathology, Boston, MA 02115, USA. campbellja@tch.harvard.edu

Journal of Immunology (Baltimore, Md. : 1950)
|January 6, 2001
PubMed
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The chemokine receptor CCR7 (CC chemokine receptor type 7) guides T cells to lymph nodes. Loss of CCR7 expression on T cells correlates with their migration to nonlymphoid tissues, indicating altered immune cell trafficking.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • CCR7 (CC chemokine receptor type 7) is crucial for T cell homing to secondary lymphoid organs via high endothelial venules (HEV).
  • CCR7 also influences lymphocyte positioning within lymphoid organs and their return from nonlymphoid tissues.
  • Understanding CCR7's role is key to deciphering immune cell trafficking and positioning.

Purpose of the Study:

  • To generate monoclonal antibodies (mAbs) against human CCR7.
  • To investigate the expression patterns of CCR7 on various T cell subsets.
  • To correlate CCR7 expression with T cell homing capabilities and tissue distribution.

Main Methods:

  • Generation of monoclonal antibodies (mAbs) specific for human CCR7.
  • Flow cytometry analysis of CCR7 expression on peripheral blood T cells and lymphocytes from nonlymphoid tissues.

Related Experiment Videos

  • Assessment of T cell subsets, including CD27 and L-selectin expression, in conjunction with CCR7 levels.

    • Main Results:

    • CCR7 is expressed on most peripheral blood T cells, including those with adhesion molecules for nonlymphoid tissue homing.
    • A subset of CD27(neg) memory CD4 T cells lacks CCR7 and L-selectin, suggesting impaired homing to secondary lymphoid organs.
    • Activated T cells often downregulate CCR7 and upregulate CXCR5 (CXC chemokine receptor type 5), influencing positioning within lymphoid organs.
    • Nonlymphoid tissues contain a significant population of CD27(neg) cells lacking CCR7.

    Conclusions:

    • CCR7 expression defines T cell populations with distinct homing potentials.
    • CCR7 downregulation is associated with migration to nonlymphoid tissues and may indicate active antigen recognition.
    • Reciprocal CCR7 and CXCR5 expression may regulate the positioning of resting versus activated T cells within lymphoid organs.