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Related Experiment Videos

Two rat brain staufen isoforms differentially bind RNA.

M Monshausen1, U Putz, M Rehbein

  • 1Institute for Cell Biochemistry and Clinical Neurobiology, University of Hamburg, Germany.

Journal of Neurochemistry
|January 9, 2001
PubMed
Summary
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Researchers identified mammalian Staufen as a protein that binds to dendritic targeting elements in microtubule-associated protein 2 (MAP2) mRNA. This protein may link RNAs to the microtubule cytoskeleton in neurons, regulating their localization.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • RNA Biology

Background:

  • Dendrites of neurons contain a subset of mRNAs, including microtubule-associated protein 2 (MAP2) mRNA.
  • A cis-acting dendritic targeting element (DTE) was recently identified in MAP2 mRNAs.

Purpose of the Study:

  • To identify trans-acting RNA-binding factors that interact with the MAP2 mRNA DTE.
  • To characterize the function of mammalian Staufen in neurons.

Main Methods:

  • Yeast tri-hybrid system to screen for RNA-binding proteins.
  • In vitro north-western assay to assess RNA-binding affinity.
  • Immunohistochemistry and ultrastructural studies in rat brain.

Main Results:

Related Experiment Videos

  • A mammalian protein homologous to Drosophila Staufen was identified as a DTE-binding protein.
  • Two rat Staufen isoforms (rStau+I6 and rStau-I6) were characterized, both binding RNA, with rStau-I6 showing stronger binding.
  • Rat Staufen was localized to neuronal somata and dendrites, accumulating along microtubules.

    • Conclusions:

    • Mammalian Staufen binds to the MAP2 mRNA DTE.
    • Staufen isoforms exhibit differential RNA-binding affinities.
    • Staufen may link dendritic RNAs to the microtubule cytoskeleton, influencing their subcellular localization in neurons.