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Related Experiment Videos

Effect of a polymeric surfactant on electron transport in HL-60 cells.

N Rapoport1, A P Marin, A A Timoshin

  • 1Department of Bioengineering, University of Utah, Salt Lake City 84112, USA. natasha@rapoport@m.cc.utah.edu

Archives of Biochemistry and Biophysics
|January 9, 2001
PubMed
Summary

Polymeric surfactants like Pluronic P-105 can reduce electron transport chain activity in mitochondria. This impacts cellular energy metabolism and the rates of energy-dependent processes in HL-60 cells.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Pharmacology

Background:

  • Mitochondrial electron transport chains (ETCs) are crucial for cellular energy production.
  • Polymeric surfactants are used in various biomedical applications.
  • Understanding their cellular effects is vital for safe application.

Purpose of the Study:

  • To investigate the impact of Pluronic P-105 on mitochondrial ETC activity in HL-60 cells.
  • To evaluate how Pluronic P-105 affects the bioreduction rates of spin probes and a drug.
  • To determine if Pluronic P-105 modulates intracellular energy metabolism.

Main Methods:

  • Utilized spin probes 16-doxylstearic acid methyl ester (16-DSME) and 5-doxylstearic acid (5-DS) to assess ETC activity.
  • Evaluated the bioreduction of Ruboxyl (Rb), a spin-labeled anthracycline drug.

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  • Employed MTT assay to corroborate findings on cell viability and metabolic activity.

    • Main Results:

    • Pluronic P-105 decreased the bioreduction rate of 16-DSME at concentrations above 0.01%, indicating reduced ETC activity.
    • The bioreduction kinetics of 5-DS became complex after Pluronic exposure.
    • Ruboxyl bioreduction decreased with increasing Pluronic concentration, suggesting impaired intracellular processes.

    Conclusions:

    • Short-term incubation with Pluronic P-105 reduces mitochondrial ETC activity in HL-60 cells.
    • Polymeric surfactants can modulate intracellular energy metabolism.
    • These modulations may affect various energy-dependent cellular processes.