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Modulation of WHSC2 expression in human endothelial cells
M Mariotti1, M Manganini, J A Maier
1Department of Preclinical Sciences-LITA Vialba, University of Milano, Via G.B. Grassi, 74, 20157, Milan, Italy.
FEBS Letters
|January 11, 2001
Summary
The WHSC2 gene, linked to Wolf-Hirschhorn syndrome, is found in endothelial cells and may act as a transcriptional repressor. HIV-1 Tat downregulates WHSC2, suggesting a role in viral-host interactions.
Area of Science:
- Genetics
- Molecular Biology
- Cell Biology
Background:
- The WHSC2 gene, located in the critical region of Wolf-Hirschhorn syndrome, is identified in endothelial cells.
- WHSC2 expression is modulated by HIV-1 Tat but not by angiogenic or pro-differentiative factors.
Purpose of the Study:
- To characterize the WHSC2 gene and its protein product.
- To investigate the cellular localization and potential function of WHSC2.
Main Methods:
- In vitro translation to determine protein molecular weight.
- Transfection of NIH-3T3 cells with whsc2-GFP to visualize nuclear translocation.
- Bioinformatic analysis to identify protein motifs (e.g., helix-loop-helix).
Main Results:
- WHSC2 encodes a 57 kDa basic polypeptide.
- The protein possesses two nuclear translocation sequences, mediating active transport to the nucleus.
- A helix-loop-helix (HLH) motif was identified in region 130-185 of WHSC2.
Conclusions:
- WHSC2 is actively transported to the nucleus.
- The presence of an HLH motif suggests WHSC2 may function as a transcriptional repressor, potentially influencing cell differentiation and cell cycle progression.