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Electron microscopic immunolocalization of basic fibroblast growth factor in peripheral nerves
1Oregon Health Sciences University, Center for Research on Occupational and Environmental Toxicology, EM/Histology Core, 3181 SW Sam Jackson Park Road, L606, Portland, OR 97201-3098, USA.
Histochemistry and Cell Biology
|January 11, 2001
Summary
Basic fibroblast growth factor (bFGF) is primarily found in Schwann cells within peripheral nerves, particularly in their nuclei. This localization suggests bFGF plays a role in maintaining and regenerating peripheral nerve function.
Area of Science:
- Neuroscience
- Cell Biology
- Peripheral Nervous System Research
Background:
- Basic fibroblast growth factor (bFGF) is well-documented in the central nervous system.
- Limited data exists on bFGF distribution and function in the peripheral nervous system (PNS).
- bFGF is known to regulate trophic and non-trophic functions.
Purpose of the Study:
- To investigate the precise localization of bFGF in normal peripheral nerves.
- To examine the presence of bFGF at the electron microscopic level.
Main Methods:
- Electron microscopy was employed to visualize bFGF.
- Immunohistochemical techniques were used for precise localization studies.
Main Results:
- bFGF was predominantly found in Schwann cells, especially within their nuclei.
- Minor bFGF labeling was observed in the myelin sheath and axon cytoplasm.
- Morphological evidence confirmed an association between bFGF expression and Schwann cells.
Conclusions:
- Schwann cells are the primary site of bFGF localization in peripheral nerves.
- The association of bFGF with Schwann cells supports its role in peripheral nerve maintenance and regeneration.