Related Experiment Video
Updated: Jul 7, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries
Published on: August 11, 2011
Genomic exploration of the hemiascomycetous yeasts: 3. Methods and strategies used for sequence analysis and
F Tekaia1, G Blandin, A Malpertuy
1Unité de Génétique Moléculaire des Levures (URA 2171 CNRS and UFR927 Univ. P.M. Curie), Institut Pasteur, Paris, France. tekaia@pasteur.fr
Abstract:
The primary analysis of the sequences for our Hemiascomycete random sequence tag (RST) project was performed using a combination of classical methods for sequence comparison and contig assembly, and of specifically written scripts and computer visualization routines. Comparisons were performed first against DNA and protein sequences from Saccharomyces cerevisiae, then against protein sequences from other completely sequenced organisms and, finally, against protein sequences from all other organisms. Blast alignments were individually inspected to help recognize genes within our random genomic sequences despite the fact that only parts of them were available. For each yeast species, validated alignments were used to infer the proper genetic code, to determine codon usage preferences and to calculate their degree of sequence divergence with S. cerevisiae. The quality of each genomic library was monitored from contig analysis of the DNA sequences. Annotated sequences were submitted to the EMBL database, and the general annotation tables produced served as a basis for our comparative description of the evolution, redundancy and function of the Hemiascomycete genomes described in other articles of this issue.
Related Concept Videos
Evolutionary Relationships through Genome Comparisons
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Genome Annotation and Assembly
Modern Molecular Taxonomy

