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Heparinase I acts on a synthetic heparin pentasaccharide corresponding to the antithrombin III binding site

G Yu1, L LeBrun, N S Gunay

  • 1Division of Medicinal and Natural Products Chemistry, University of Iowa, Iowa City, IA 52242, USA.

Thrombosis Research
|January 12, 2001
PubMed

A synthetic pentasaccharide, containing an intact antithrombin III (ATIII) binding site that is in clinical studies a specific antifactor Xa agent, serves as a substrate for a heparin lyase (heparinase I, EC 4.2.2.7) from Flavobacterium heparinum. Heparinase I, currently being assessed as a heparin reversal agent, also reverses the antifactor Xa activity of this synthetic pentasaccharide by breaking it down to inactive disaccharide and trisaccharide products.

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