Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Training software for high-performance liquid chromatography.

J C Reijenga1

  • 1Laboratory of Instrumental Analysis, Eindhoven University of Technology, The Netherlands. j.c.reijenga@tue.nl

Journal of Chromatography. A
|January 12, 2001
PubMed
Summary

A new computer simulation program models reversed-phase high-performance liquid chromatography (HPLC) for training. It uses a two-parameter retention model to predict compound behavior with varying organic modifiers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antioxidants reduce oxidative stress in claudicants.

The Journal of surgical research·2001
Same author

Determination of methacrylic acid in the drain of a biotrickling filter using isotachophoresis and capillary zone electrophoresis.

Journal of chromatography. A·1999
Same author

Determination of free radical reaction products and metabolites of salicylic acid using capillary electrophoresis and micellar electrokinetic chromatography.

Journal of chromatography. B, Biomedical sciences and applications·1998
Same author

Chiral interactions in capillary zone electrophoresis: computer simulation and comparison with experiment.

Electrophoresis·1997
Same author

Quality control of histamine and methacholine in diagnostic solutions with capillary electrophoresis.

Journal of chromatography. A·1996
Same author

Training software for electrophoresis.

Electrophoresis·1995

Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Computational Chemistry

Background:

  • High-performance liquid chromatography (HPLC) is a crucial separation technique.
  • Accurate prediction of retention behavior is essential for method development and training.
  • Existing simulation tools may lack comprehensive parameter variability.

Purpose of the Study:

  • To develop a user-friendly computer simulation program for reversed-phase HPLC training.
  • To create a robust two-parameter retention model adaptable to various experimental conditions.
  • To provide detailed output including dispersion data for enhanced learning.

Main Methods:

  • Development of a computer simulation program for reversed-phase HPLC.
  • Reduction of experimental retention data for 75 organic compounds into a two-parameter model.

Related Experiment Videos

  • Inclusion of variable organic modifier content (acetonitrile, methanol, tetrahydrofuran).
  • Simulation of both isocratic and programmed solvent compositions.
  • Integration of common HPLC detector types (UV-Vis, refractive index) and variable instrument parameters.

    • Main Results:

    • A functional simulation program accurately models experimental retention values.
    • The two-parameter retention model effectively predicts compound behavior across different modifier concentrations.
    • The program accommodates a wide range of experimental variables, including solvent composition and instrument settings.
    • Detailed dispersion data is generated for comprehensive analysis.

    Conclusions:

    • The developed simulation program serves as an effective training tool for reversed-phase HPLC.
    • The retention model provides a simplified yet accurate approach to predicting chromatographic behavior.
    • The program's flexibility in parameter adjustment enhances its utility for method development and educational purposes.