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Regulation of a wheat actin-depolymerizing factor during cold acclimation.

F Ouellet1, E Carpentier, M J Cope

  • 1Département des Sciences Biologiques, Université du Québec à Montréal, Case Postale 8888, Succursale Centre-ville, Montréal, Québec, Canada H3C 3P8.

Plant Physiology
|January 12, 2001
PubMed
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Wheat plants possess a TaADF gene crucial for freezing tolerance. This actin-depolymerizing factor (ADF) protein accumulates in cold-acclimated plants, enhancing their ability to withstand freezing temperatures.

Area of Science:

  • Plant molecular biology
  • Biochemistry
  • Cryobiology

Background:

  • The wheat (Triticum aestivum) TaADF gene expression correlates with freezing tolerance.
  • The gene encodes a protein homologous to actin-depolymerizing factor (ADF)/cofilin family members.

Purpose of the Study:

  • Characterize the recombinant TaADF protein.
  • Investigate the role of TaADF in plant cold acclimation and freezing tolerance.

Main Methods:

  • Recombinant protein expression and purification.
  • In vitro actin-depolymerizing factor assays.
  • In vitro phosphorylation studies using wheat kinase.
  • Western-blot analysis for TaADF expression in Gramineae species.

Main Results:

Related Experiment Videos

  • TaADF sequesters actin, prevents nucleotide exchange, and depolymerizes actin.
  • TaADF is phosphorylated by a wheat 52-kD kinase, whose activity is modulated by low temperature.
  • TaADF accumulates in cold-acclimated Gramineae, with higher levels in freezing-tolerant wheat cultivars.
  • TaADF accumulation is regulated by genes on chromosome 5A, associated with cold hardiness.

Conclusions:

  • The induction of active TaADF during cold acclimation correlates with increased freezing tolerance.
  • TaADF may be essential for cytoskeletal rearrangements during low-temperature exposure, enhancing freezing tolerance.