Related Experiment Videos
Genomewide function conservation and phylogeny in the Herpesviridae
1Wohl Virion Centre, Department of Immunology and Molecular Pathology, University College London, London W1T 4JF, UK.
Genome Research
|January 13, 2001
Summary
Researchers analyzed 19 herpesvirus genomes, identifying 243 protein families and conserved functions. This provided new insights into the evolutionary relationships of ungulate and murine gammaherpesviruses.
Area of Science:
- Virology
- Bioinformatics
- Genomics
Background:
- Herpesviridae are double-stranded DNA viruses with numerous sequenced genomes.
- Understanding protein homology is crucial for viral evolution studies.
Purpose of the Study:
- To construct herpesvirus protein homologous families.
- To classify proteins into functional groups.
- To infer viral phylogeny using conserved functions.
Main Methods:
- Extracted protein sequences from 19 herpesvirus genomes.
- Employed sequence comparison and clustering for protein family construction.
- Assigned functions and classified families into broad groups.
- Built phylogenetic profiles and trees based on homologous families.
Main Results:
- Clustered 1692 proteins into 243 multiprotein families and 196 singletons.
- Assigned predicted functions to homologous families.
- Constructed genomewide phylogenetic trees that align with molecular-sequence trees.
- Gained insights into ungulate and murine gammaherpesvirus phylogeny.
Conclusions:
- Established a comprehensive framework for herpesvirus protein family analysis.
- Demonstrated the utility of phylogenetic profiles for understanding viral evolution.
- Advanced the understanding of gammaherpesvirus phylogeny and conserved functions.