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Twelve-hour PCR-based method for detection of Salmonella spp. in food
R Ferretti1, I Mannazzu, L Cocolin
1Dipartimento di Biotecnologie Agrarie ed Ambientali, 60100 Ancona, Italy. anmicro@popcsi.unian.it
Applied and Environmental Microbiology
|February 7, 2001
Abstract:
A PCR-based method for the detection of Salmonella spp. in food was developed. The method, set up on typical salami from the Italian region of Marche, is sensitive and specific and shows excellent correlation with the conventional method of reference when naturally contaminated foods are analyzed. Moreover, it can be easily performed within a maximum of 12 h from food sampling, thus allowing prompt detection of Salmonella spp. in the food stocks analyzed.