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Goldmann applanation tonometry in the conscious rat
1Eye Research Fund Laboratory, Department of Physiology, Medical School, University of Michigan, Ann Arbor 48104, USA. becohan@umich.edu
Investigative Ophthalmology & Visual Science
|February 7, 2001
Summary
Modified Goldmann tonometers can now measure intraocular pressure (IOP) in conscious rats. This advancement allows for more accurate IOP assessment in rat models, aiding glaucoma research.
Area of Science:
- Ophthalmology
- Animal Models
- Physiological Measurement
Background:
- Intraocular pressure (IOP) is a critical factor in ocular health.
- The Goldmann applanation tonometer is the standard for human IOP measurement.
- Measuring IOP in conscious rats has been challenging.
Purpose of the Study:
- To modify the Goldmann applanation tonometer for IOP measurement in conscious rats.
- To validate the modified tonometer's accuracy in a live animal model.
Main Methods:
- Goldmann tonometers were modified with reduced biprism angles and weights.
- Modified tonometers were calibrated against manometric IOP measurements in anesthetized rats.
- The modified tonometer was used to measure IOP in conscious, unsedated rats.
Main Results:
- The modified Goldmann tonometer successfully measured IOP in conscious rats.
- A mean IOP of 15.5 +/- 0.6 mm Hg was recorded.
- Repeated applanations were necessary to overcome an initial artifactual decline in readings.
Conclusions:
- The Goldmann applanation tonometer can be effectively modified for use in conscious rats.
- This modification enables the application of a standard human IOP measurement tool to rat models.
- This advancement is expected to enhance the utility of rats in IOP and glaucoma research.
