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A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Quantitative detection of hepatitis C virus RNA by light cycler PCR and comparison with two different PCR assays
M Schröter1, B Zöllner, P Schäfer
1Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Hamburg-Eppendorf, 20246 Hamburg, Germany. mschroet@uke.uni-hamburg.de
Journal of Clinical Microbiology
|February 7, 2001
Abstract:
The new Light Cycler technology was adapted to the detection of hepatitis C virus (HCV) RNA in clinical samples. Sera from 81 patients were tested by Light Cycler PCR, AMPLICOR HCV Monitor assay, and in-house PCR. Our data demonstrate that Light Cycler is a fast and reliable method for the detection and quantitation of HCV RNA.

