In vivo microdialysis for nonapeptides in rat brain--a practical guide

Microdialysis provides a direct approach to monitor changes in interneuronal communication by monitoring the fluctuation of local, extracellular concentrations of potential neurotransmitters/neuromodulators. The present article is based on more than 10 years experience in performing microdialysis experiments in freely moving animals with inexpensive self-made microdialysis probes and accessories for monitoring of intracerebral neuropeptide release. On the basis of this experience, we provide a guide for the construction of different types of microdialysis probes and their application. Furthermore, we give information about organizing and performing a microdialysis experiment that can easily be adapted to fit individual applications needs. Finally, on the basis of theoretical background information advantages as well as limitations of the microdialysis technique are discussed with the intent to provide help to potential users for designing an appropriate microdialysis experiment.