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Molecular characterization of rabbit renal epithelial calcium channel
1Department of Integrative Biology, Physiology, and Pharmacology, Houston Medical School, University of Texas, 6431 Fannin, Houston, Texas, 77030, USA. min.zhang@uth.tmc.edu
Abstract:
PCR and antisense oligodeoxy-nucleotide (ODN) blocking were used to identify a calcium (Ca) channel in rabbit proximal tubule (PT) cells. The subcloned Ca channel is identical to the rabbit cardiac Ca channel (alpha(1)) except a 33 base deletion at the fourth S3-S4 linker in PT cells. Anti-sense ODN treatment (18 h) inhibited 73 and 44% of Ca influxes induced by hypoosmotic stress (220 Osm) and by 1-oleoyl-2-acetyl-sn-glycerol (5 microM), respectively. The results indicate that the subcloned channel is a spliced variant of the cardiac Ca channel and that it plays a critical role in regulation of Ca signaling in these cells.